The course is designed to provide the participants with key concepts of the microbiological monitoring of laboratory rodents and their environment. The program includes an overview on traditional and emerging pathogenic agents, the description of different screening techniques for animals and biologicals. In addition, the necessary assessments in relation to the factors that influence the approach of the screening plan, from the housing system in use to the identification of the microbiological unit will be discussed. An open session will provide opportunities to discuss the practical challenges related to the management of a microbiological monitoring program.